In vitro and in vivo antifungal activity of synthetic pure isothiocyanates against Sclerotinia sclerotiorum


KURT Ş., Güneş U., SOYLU E. M.

Pest Management Science, cilt.67, sa.7, ss.869-875, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 67 Sayı: 7
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1002/ps.2126
  • Dergi Adı: Pest Management Science
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.869-875
  • Anahtar Kelimeler: Antifungal activity, Isothiocyanates, Sclerotinia sclerotiorum
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

BACKGROUND: Isothiocyanates (ITCs) released by the enzymatic hydrolysis of glucosinolates in the Brassicaceae are potentially useful for controlling fungal pathogens. In vitro activity of pure ITCs against Sclerotinia sclerotiorum (Lib.) de Bary was studied by adding them to glass filters in petri dishes and dissolving them in the growing media. RESULTS: Methyl, allyl and benzyl ITCs were the most fungitoxic of the compounds in bioassays with S. sclerotiorum isolate Ss31. In the volatile phase, mycelial growth was completely inhibited by these three compounds. Aromatic ITCs were less toxic in the petri dishes but were more toxic than aliphatic ITCs when dissolved in the agar. Benzyl ITC exhibited the highest inhibitory effect on sclerotial germination, with an EC50 value of 75.1 μmol L-1. Butyl and benzyl ITCs reduced apothecial production of S. sclerotiorum by 92.5% at the highest concentration. In in vivo assay, only allyl and 2-phenylethyl ITCs reduced disease incidence (by 76.7 and 70% respectively) at low concentrations. CONCLUSION: Sclerotinia sclerotiorum in the soil might be suppressed by the higher concentrations of allyl and benzyl ITCs released from decomposition of Brassica juncea, B. carinata, B. nigra and Sinapis spp. © 2011 Society of Chemical Industry.