Research Information System
Mustafa Kemal Üniversitesi Tıp Dergisi, vol.12, no.43, pp.73-76, 2021 (Peer-Reviewed Journal)
Objective: Appetite is affected by the metabolic profile, infections and nutritional shape in domestic animals. Regulation of appetite involves many complex physiological processes. Ghrelin is well known appetite-enhancing hormone. The appetite is also managed by the lateral hypothalamus. On the other hand, the ventromedial nucleus is most commonly associated with satiety. Brotizolam, member of the 1,4-benzodiazepines derivative, is blocking the activity of the ventromedial hypothalamus and used to increase appetite in domestic animals. The aim of this study was to investigate the impact of brotizolam on serum ghrelin levels, enhancer appetite hormone.Methods: A total of 16 mice were equally assigned to two groups as control (0.9% NaCl, IP) and brotizolam (2 μg/kg, IP). Blood samples was collected 30 min after the administration in both groups. Serum ghrelin levels were determined by enzyme linked immunoassay.Results: There was a significance differences between control and brotizolam groups (P<0.001). The results of the study showed that the administration of brotizolam increased serum ghrelin levels.Conclusion: Brotizolam, which suppresses ventromedial hypothalamus and satiety, can activate appetite by activation of the lateral hypothalamus and increase in ghrelin levels.In addition, ghrelin may be used as an endocrine marker of appetite.