Cryopreservation of scaly carp (Cyprinus carpio) sperm: Effect of different cryoprotectant concentrations on post-thaw motility, fertilization and hatching success of embryos


YAVAŞ İ., Bozkurt Y., Yildiz C.

Aquaculture International, cilt.22, sa.1, ss.141-148, 2014 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 22 Sayı: 1
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1007/s10499-013-9698-6
  • Dergi Adı: Aquaculture International
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.141-148
  • Anahtar Kelimeler: Cryopreservation, Cryoprotectant, Cyprinus carpio, Fertilization, Hatching, Motility, Scaly carp
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

The aim of the present study was to determine the effect of various cryoprotectants on post-thaw sperm quality and fertilizing capacity of cryopreserved scaly carp (Cyprinus carpio) semen. The present study focused on freezing of scaly carp sperm utilizing a practical and inexpensive protocol for aquaculture. Semen was diluted with Kurokura's extender composing 3.6 g/l NaCl, 10 g/l KCl, 0.22 g/l CaCl2, 0.08 g/l MgCl2 and 0.2 g/l NaHCO3. The extender contained three different cryoprotectants (DMSO, DMA and egg yolk) at ratios of 5, 10 and 15 %. Semen was placed into 0.25-ml straws and exposed to liquid nitrogen vapor (-120 °C) using an insulated box with an adjustable tray for 10 min and then plunged into liquid nitrogen (-196 °C) tank. The thawing process was performed in a water bath at 40 °C for 10 s. The results indicated that type of cryoprotectants and their concentrations are rather effective in scaly carp sperm cryopreservation on post-thaw sperm quality, while they are very important in order to obtain high fertilization rates. The highest fertilization rate was determined as 96.4 ± 0.15 % with 15 % egg yolk, while the highest hatching rate was determined as 99.3 ± 0.80 with 15 % DMA. In conclusion, the applied cryopreservation method for scaly carp sperm is suitable to fertilize high amounts of eggs. © 2013 Springer Science+Business Media Dordrecht.