Some properties of thermostable xylanase from an Aspergillus niger strain

Coral G., ARIKAN B., Ünaldi M., Güvenmez H. K.

Annals of Microbiology, cilt.52, sa.3, ss.299-306, 2002 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 52 Sayı: 3
  • Basım Tarihi: 2002
  • Dergi Adı: Annals of Microbiology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.299-306
  • Anahtar Kelimeler: Aspergillus niger, Xylanase
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

A thermostable xylanase was isolated from an Aspergillus niger wild type strain in a liquid Czapek Dox medium, containing oat spelts xylan as the sole carbon source. The molecular mass of the enzyme was estimated to be about 36 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimum pH for activity was found to be 7.5. The temperature optimum of the enzyme was found to be 60 °C at pH 7.5. The enzyme remained stable up to 100 °C, yet lost about 50% of its activity after 15 min at this temperature.