Some properties of thermostable xylanase from an Aspergillus niger strain

Coral G., ARIKAN B., Ünaldi M., Güvenmez H. K.

Annals of Microbiology, vol.52, no.3, pp.299-306, 2002 (SCI-Expanded, Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 52 Issue: 3
  • Publication Date: 2002
  • Journal Name: Annals of Microbiology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.299-306
  • Keywords: Aspergillus niger, Xylanase
  • Hatay Mustafa Kemal University Affiliated: Yes

Abstract

A thermostable xylanase was isolated from an Aspergillus niger wild type strain in a liquid Czapek Dox medium, containing oat spelts xylan as the sole carbon source. The molecular mass of the enzyme was estimated to be about 36 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimum pH for activity was found to be 7.5. The temperature optimum of the enzyme was found to be 60 °C at pH 7.5. The enzyme remained stable up to 100 °C, yet lost about 50% of its activity after 15 min at this temperature.