Research Information System
Jordan Journal of Biological Sciences, vol.15, no.1, pp.133-137, 2022 (Scopus)
Background & objectives: Lactobacillus species or their metabolites (such as lactic acid or bacteriocin) are known to protect the vagina from urogenital infections and to maintain vaginal hygiene. The goal of the current study was to identify lactobacilli species for a possible probiotic and postbiotic perspective. In Mostaganem city (Algeria), an effective molecular detective technique for lactobacilli in the vagina of women is seriously lacking. Therefore, our current studies promise to contribute to molecular diagnostic. Materials and Methods: 37 samples of vaginal lactobacilli from 32 healthy women were isolated. By using polymerase chain reaction (PCR), strains were identified. For identification, genus specific, group specific and species-specific PCR protocols were implemented. Results: All isolates were confirmed as spp. Lactobacillus. All 37 isolates belonged to Group IV of the Lactobacillus genus and were further divided into two L. reuteri and L. salivarius species. Result interpretation & conclusion: In this study, the prevalent species in vagina were L. reuteri (70, 27%), followed by L. salivarius (29,73%). The probiotic potential of L. salivarius and L. reuteri from vaginal origin has not been fully exploited yet but studies to date have shown that these two species may be reliable candidates for use as new probiotics or as 'postbiotic' metabolites.