Keratinolytic activity of Streptomyces strain BA7, a new isolate from Turkey
Annals of Microbiology, cilt.53, sa.1, ss.85-93, 2003 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 53 Sayı: 1
- Basım Tarihi: 2003
- Dergi Adı: Annals of Microbiology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.85-93
- Anahtar Kelimeler: Feather, Keratinase, Streptomyces
- Hatay Mustafa Kemal Üniversitesi Adresli: Evet
Özet
A wild type Streptomyces strain BA7 which was isolated from soil, showed a high keratinolytic activity when cultured on native feather medium. Optimum keratinolytic activity was observed at 50°C and pH 8.5 when using fluid supernatant obtained from aerated culture of this organism. The keratinolytic activity was completely stable (100%) between 30 and 60°C. The molecular weight of crude enzyme was estimated by SDS-PAGE about 44000 Da. The degradation of intact feathers by Streptomyces sp. BA7 keratinase was obtained after 24 h of incubation at 50°C. Keratinolytic activity was partially inhibited by 1,10-phenanthroline, but slightly inhibited by CaCl2, ZnCl2, and SDS. In addition keratinolytic activity was enhanced by DMSO, EDTA, Triton-X 100 and sodium sulphite. Streptomyces strain BA7 could be a useful in biotechnology in therms of valorization of keratin-containing wastes or in the leather industry.