Flowerlike hybrid horseradish peroxidase nanobiocatalyst for the polymerization of guaiacol

GÖKTÜRK E.

Turkish Journal of Chemistry, cilt.44, sa.5, ss.1285-1292, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 5
  • Basım Tarihi: 2020
  • Doi Numarası: 10.3906/kim-2005-32
  • Dergi Adı: Turkish Journal of Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.1285-1292
  • Anahtar Kelimeler: Guaiacol, Horseradish peroxidase, Hybrid nanoflowers, Polymerization
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

In this study, the catalytic activity and stability of flowerlike hybrid horseradish peroxidase (HRP) nanobiocatalyst (HRPCu2+) obtained from Cu2+ions and HRP enzyme in the polymerization reaction of guaiacol were analyzed. We demonstrated that HRP-Cu2+and hydrogen peroxide (H2O2) initiator showed significantly increased catalytic activity and stability on the polymerization of guaiacol compared to that of free HRP enzyme. Poly(guaiacol) was observed with quite high yields (88%) and molecular weights (38,000 g/mol) under pH 7.4 phosphate-buffered saline (PBS) conditions at 60 °C with 5 weight% of HRP-Cu2+loading. HRP-Cu2+also shows very high thermal stability and works even at 70 °C reaction temperature; free HRP enzyme denatures at that temperature. Furthermore, HRP-Cu2+provided considerable repeated use and showed some degree of catalytic activity, even after the fourth recycle, in the polymerization of guaiacol.