Identification of Fungal Pathogens Causing Fruit Drop in Citrus Orchards and Antifungal Effects of Some Compounds


UYSAL A., KURT Ş., SOYLU S., KARA M., SOYLU E. M.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, sa.6, ss.1401-1413, 2024 (ESCI) identifier identifier

Özet

This study identified the fungal agents causing fruit drop in citrus orchards in Hatay province and investigated the in vitro antifungal effects of boron compounds and pyroligneous acid (PA) against these pathogens. Thirty isolates were obtained from orange, mandarin, lemon, and grapefruit orchards in the Erzin, D & ouml;rtyol, Arsuz, and Samanda & gbreve; districts of Hatay. Pathogen isolation was performed on the PDA oxysporum, Alternaria alternata, Colletotrichum gloeosporioides, Colletotrichum karsti, and Diaporthe foeniculina as the disease agents responsible for fruit drop. Pathogenicity tests confirmed all isolates as pathogenic. Colletotrichum spp. exhibited the highest virulence with 92.6-88.9%, followed by D. foeniculina at 66.7%, F. oxysporum at 55.6%, and A. alternata at 44.4%. The antifungal effects of boron compounds and various concentrations of PA (0.03%, 0.05%, 0.07%, 0.09%, 0.1%, 0.12%, 0.15%, 0.3%, 0.5%, 0.7%, 1.0%, 1.5%; w/v) on the mycelial growth and conidial germination of five fungal pathogen isolates were investigated in vitro. Mycelial growth was completely inhibited by boric acid (0.12%), borax (0.1%), ethidote-67 (0.1%), and PA (1.5%). Additionally, boric acid (0.15%), borax (0.12%), Etidot-67 (0.12%), and PA (1.5%) significantly inhibited conidial germination. The EC50 values for mycelial growth ranged from 0.059% to 0.69%, and for conidial germination, from 0.065% to 0.82%. This is the first study investigating the antifungal activity of boron compounds and PA against fungal pathogens causing citrus fruit drop in T & uuml;rkiye.