Protective effects of N-acetylcysteine on triamcinolone acetonide-induced lens damage in rats


Tuzcu E. A., Tuzcu K., Basarslan F., Motor S., Coskun M., Keskin U., ...Daha Fazla

Cutaneous and Ocular Toxicology, cilt.33, sa.4, ss.294-298, 2014 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 4
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3109/15569527.2013.857679
  • Dergi Adı: Cutaneous and Ocular Toxicology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.294-298
  • Anahtar Kelimeler: Cataract, Intravitreal triamcinolone acetonide injection, N-acetylcysteine, Oxidative status
  • Hatay Mustafa Kemal Üniversitesi Adresli: Evet

Özet

Objective: To examine the relationship of cataract forming effect of intravitreal triamcinolone acetonide (IVTA) injection with oxidative status and the effect of N-acetylcysteine (NAC) on these alterations. Materials and methods: Twenty-six Wistar-Albino rats were included in the study. Rats were assigned into four groups as follows: intravitreal saline injection group (controls); IVTA injection group; IVTA+intraperitoneal NAC injection group (IVTA+NAC); and intraperitoneal NAC injection group (NAC). Triamcinolone acetonide was intravitreally injected at a dose of 1mg. NAC was intraperitoneally injected at a dose of 150μg/g body weight. Animals were sacrificed and lens specimens were analyzed for levels of malondialdehyde (MDA) and protein carbonyl (PC) and activities of glutathione (GSH) and glutathione peroxidase (GSH-Px). Results: We found that the MDA and PC levels of lenses were increased in the IVTA group (p<0.01). It was seen that GSH and GSH-Px in lenses were decreased in the IVTA group (p<0.01). NAC administration significantly ameliorated these changes in the IVTA+NAC group (p<0.05). Conclusion: These results indicate that the NAC produces a protective mechanism against IVTA-induced cataract and suggest a role of oxidative stress in pathogenesis.