Radioprotective profile of urtica dioica L. Seed extract on oxidative dna-damage in liver tissue and whole blood of radiation-administered rats

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Çakır T., Yıldızhan K., Huyut Z., Uyar A., Arıhan O.

Brazilian Journal of Pharmaceutical Sciences, vol.56, pp.1-9, 2020 (SCI-Expanded, Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 56
  • Publication Date: 2020
  • Doi Number: 10.1590/s2175-97902019000318382
  • Journal Name: Brazilian Journal of Pharmaceutical Sciences
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Page Numbers: pp.1-9
  • Keywords: Antioxidant, DNA damage, Radioprotective, Urtica
  • Open Archive Collection: AVESIS Open Access Collection
  • Hatay Mustafa Kemal University Affiliated: No

Abstract

It was aimed to investigate the radioprotective activity of Urtica dioica L. seed extract (UDSE) in the whole blood and liver of radiation-administered rats, both biochemically and immunohistochemically. 32 rats were divided into 4 groups (n:8). Control group (C): no administration for 10 days. Radiation group (IR): fed pellets for 10 days after exposure to radiation. Radiation + UDSE (IR+UDSE) group: exposed to radiation and fed UDSE for 10 days. UDSE group (UDSE): fed UDSE for 10 days. Radiation (5Gy) was given as a single fraction. 8-hydroxy-2-deoxyguanosine (8-OHdG) and deoxyguanosine (dG) levels were analyzed by biochemical method and glutathione peroxidase 1 (GPx-1) analyses were performed by immunohistochemical method in the liver and blood tissues of the rats. The increased 8-OHdG rates and decreased GPx-1 immunoreactivity was observed in the IR group. Those parameters were ameliorated in the IR+UDSE group when compared to the IR group. UDSE is likely to be a valuable radioprotector against the harmful effects of radiation.