Akademik Veri Yönetim Sistemi
Hacettepe Journal of Biology and Chemistry, cilt.42, sa.4, ss.499-504, 2014 (Hakemli Dergi)
Metal toxicity causes oxidative stress in fish. This situation is a potential risk factor for humans and other living feeding on contaminated fish. In this study, the inhibition effects of heavy metals on carbonic anhydrase enzyme from the corb fish gill were investigated. The carbonic anhydrase enzyme was purified from gill of corb fish with a specific activity of 2093,9 EUmg−1 and 86,51% yield and approximately 160 fold using Sepharose 4B–L-tyrosine sulfanilamide affinity chromatography method. SDS–polyacrylamide gel electrophoresis showed a single band corresponding to a molecular weight of approximately 30,8 kDa. Inhibitory effects of metals $(Ag^{+}, Cu^{2+}, Pb^{2+}, Zn^{2+}, Cd^{2+}, Ni^{2+})$ on CA activity were determined at different concentrations using thehydratase method under in vitro conditions. Consequently, in vitro inhibition rank order was determined as $Ag^{+>} Cu^{2+}> Pb^{2+}> Zn^{2+} > Cd^{2+}> Ni^{2+}$. From these results, we showed that $Ag^+$ is the most potent inhibitor of CA enzyme.