Akademik Veri Yönetim Sistemi
Çağlayan K., Güler G P., Gazel M., Elçiçek M., Yegül M., Tanrıver E.(Yürütücü)
TAGEM Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü Projesi, 2018 - 2022
Sharka, caused by plum pox virus (PPV), is one of the most important infectious disease of peach [P. persica (L.) Batsch] and other Prunus species. PPV has been present in Turkey since 1968 however, contamination level is restricted in some regions depending on PPV strain. PPV-D and PPV-T strains were identified mostly in residential gardens, whereas PPV-M was mostly detected in the commercial orchards. PPV-T was found to be dominant in the Turkish PPV pool. In areas where PPV is present, the cultivation of highly resistant (tolerant) genotypes is the only possibility to alleviate this problem however, no immune or resistant cultivars in peach germplasm were reported. The purpose of the research was to identify genotypes that are tolerant or resistant to the virus and to monitor the manner in which the resistance is transmitted to hybrid descendants by intergeneric hybridization between peach and other Prunus species. The material employed consisted of J.H.Hale, Cresthaven Flored, Persicum-Tan, UFO-3, P.davidiana P1908, Fantasia, Persicum-ATEŞ, Pitarina-CS46, Crimson baby, Patrones-ARDA, Garnem and GF 677. The hybridization studies were conducted during 2018-2020, F1 interspecific hybrids have been created by cross pollination between resistant or partial resistant cultivars well adapted to Turkish ecological conditions. The varieties identified as having higher resistance to the attack of PPV (Stark Early Orange, Harcot P. Dulcis, P. Davidiana and Ferraduel) were used in interspecific hybridisations for the selection of hybrids to which this characteristic was transmitted. The F1 individuals are grown under screenhouse to keep them away from PPV infection and also aphid infestation. A total of 7.800 individuals from hybrid families were screened for PPV resistance. The screening included observations for Sharka symptoms, DAS-ELISA and RT-PCR analysis, genotyping by using sets of molecular markers located in the locus linked to PPV resistance in the apricot genome (Marker Assisted Selection – MAS) and phenotyping by biological indexing and re-testing by RT-PCR. According to the MAS results from screening by two sets of molecular markers, 17 individuals (0.3 %) of all analysed individuals, originating from 5418 hybrid families were genotyped as resistant. On the base of the screening data for resistance and some agronomical evaluations of the fruits, few hybrids from the 17 crosses have been artificially inoculated for further scoring. Phenotyping studies are still under investigation.
Keywords: Plum pox virus-M (PPV-M) resistance, peach, P. davidiana, P. dulcis